AGTII Pancreatic Cancer Therapeutic Lentivirus, AG1102: Killing of pancreatic tumor cells in vitro

Monolayers of pancreatic cancer cells in culture were exposed for 8 hours  to AG1102 therapeutic lentivirus or Control virus, followed by medium change.  Phase contrast photographs of representative cultures of AG1102 Treated (left) or Control (right) at four days post-treatment are shown below.

AG1102 Treated	Control

Observations:
AG1102-Treated cells detached from the substrate.  In addition, death by cellular necrosis was observed as inconsistent size and reduced birefringence of the treated cells.  Cell metabolic measurement confirmed cell death in treated samples (not shown).

In contrast, Control cells grew steadily from initial seeding.  After cells grew to confluence additional cells grew on top of the initial monolayer.  Control sample cells maintained birefringence and size. 

In-vivo experiments (similar to those performed for the Prostate Cancer therapeutic) are planned for later this year.